Figure 1: Western Blot - Anti-IDH2 Antibody (A15584)
Western blot analysis of Jurkat, using Anti-IDH2 Antibody (A15584) at 1:600 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Figure 2: Western Blot - Anti-IDH2 Antibody (A15584)
Western blot analysis of various lysates, using Anti-IDH2 Antibody (A15584) at 1:600 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-IDH2 Antibody (A15584) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Figure 6: Western Blot - Anti-IDH2 Antibody (A15584)
Immunoprecipitation analysis of 200µg extracts of MCF7 cells using 1µg of Anti-IDH2 Antibody (A15584). This Western blot was performed on the immunoprecipitate using Anti-IDH2 Antibody (A15584) at a dilution of 1:1000.
Chromatin immunoprecipitation (ChIP) analysis of extracts of 293T cell line, using Anti-IDH2 Antibody (A15584) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
