Figure 1: Western Blot - Anti-Insulin Degrading Enzyme/IDE Antibody (A83088)
Insulin Degrading Enzyme/IDE expression in K562 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Insulin Degrading Enzyme/IDE Antibody (A83088) at 0.1µg/ml and detected by chemiluminescence.
Insulin Degrading Enzyme/IDE expression in Human Lung analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Insulin Degrading Enzyme/IDE Antibody (A83088) at 2.5µg/ml and revealed with alkaline phosphatase (AP).
Insulin Degrading Enzyme/IDE expression in Human Breast analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Insulin Degrading Enzyme/IDE Antibody (A83088) at 2.5µg/ml and revealed with alkaline phosphatase (AP).