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ID4 Cell Based Kit Elisa (A103342)

Nom du produit
ID4 Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This ID4 Cell Based ELISA Kit allows for the detection of ID4 and the effects that certain stimulation conditions have on ID4 expression in different cell lines. Qualitative determination of ID4 concentration is achieved by an indirect ELISA format. In essence, the ID4 is captured by Anti-ID4 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this ID4 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
bHLHb27, Class B basic helix-loop-helix protein 27, DNA binding protein inhibitor ID 4, DNA binding protein inhibitor ID4, DNA-binding protein inhibitor ID-4, ID 4, ID4_HUMAN, IDB4, Inhibitor of DNA binding 4, Inhibitor of DNA binding 4 dominant negative helix loop helix protein
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - ID4 Cell Based ELISA Kit (CB5896) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - ID4 Cell Based ELISA Kit (CB5896)
Western blot analysis of extracts from HepG2 cells, using Anti-ID4 Antibody. The lane on the right is treated with the synthesized peptide.
Protocol Diagram - ID4 Cell Based ELISA Kit (CB5896) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: ID4 Cell Based ELISA Kit (CB5896)
A diagram showing the protocol for ID4 Cell Based ELISA Kit.
Protocol Summary - ID4 Cell Based ELISA Kit (CB5896) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: ID4 Cell Based ELISA Kit (CB5896)
A summary of the protocol for ID4 Cell Based ELISA Kit.
Western Blot - Control Antibody - ID4 Cell Based ELISA Kit (CB5896) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - ID4 Cell Based ELISA Kit (CB5896)
The mouse monoclonal antibody to GAPDH is used as a positive control inID4 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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