Anti-ID1 Anticorps [PCRP-ID1-2F11] (A278240)

Name: Anti-ID1 Antibody [PCRP-ID1-2F11] - BSA and Azide free
Brand: Antibodies.com
SKU: A278240
Price: 525.0 USD
Availability: InStock

Cliquez pour ouvrir le point de vue élargi

$525

Anticorps monoclonal [PCRP-ID1-2F11] de souris contre ID1 pour ELISA, IP, Flow Cytometry et IF.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Alternative Formats and ConjugatesAlternative Formats

Unconjugated

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 4-6 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Mots clés :

Anticorps Monospécifique

Spécifications

Nom du produit

Anti-ID1 Antibody [PCRP-ID1-2F11] - BSA and Azide free

Description du produit

Mouse monoclonal [PCRP-ID1-2F11] antibody to ID1.

Spécificité

Members of the Id family of basic helix-loop-helix (bHLH) proteins include Id1, Id2, Id3 and Id4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The Id-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since Id1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, the Id protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation. It has been shown that expression of each of the Id proteins is strongly dependent on growth factor activation and that reduction of Id mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation.

Applications

ELISA, IP, Flow Cytometry, IF

Recommander des dilutions

IP: 1-2µg / 100-500µg proteins, Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml

Reactivité

Human

Immunogène

Recombinant fragment, around amino acids 5-150, of human ID1 protein. The exact sequence is proprietary.

Hôte

Mouse

Clonalité

Monoclonal

Clone

PCRP-ID1-2F11

Isotype

IgG1

Conjuguer

Unconjugated

Purification

Protein A/G chromatography.

Concentration

1 mg/ml

Masse moléculaire

16.13 kDa

Forme du produit

Liquid

Formulation

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes générales

This monoclonal antibody is also available in a different formulation with BSA and Sodium Azide - Anti-ID1 Antibody [PCRP-ID1-2F11] (A277652).

Synonymes

bHLHb24, Class B basic helix-loop-helix protein 24, dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein), DNA binding protein inhibitor ID 1, DNA binding protein inhibitor ID1, DNA-binding protein inhibitor ID-1, Dominant negative helix loop helix protein, ID, ID 1, ID1_HUMAN, Inhibitor of Differentiation 1, Inhibitor of DNA binding 1, inhibitor of DNA binding 1, dominant negative helix-loop-helix protein

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

Cliquez pour ouvrir le point de vue élargi

Figure 1: Flow Cytometry - Anti-ID1 Antibody [PCRP-ID1-2F11] - BSA and Azide free (A278240)

Flow cytometric analysis of PFA-fixed HeLa cells using Anti-ID1 Antibody [PCRP-ID1-2F11] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Isotype Control (Red).

Cliquez pour ouvrir le point de vue élargi

Figure 2: Immunofluorescence - Anti-ID1 Antibody [PCRP-ID1-2F11] - BSA and Azide free (A278240)

Immunofluorescent analysis of PFA-fixed HeLa cells stained with Anti-ID1 Antibody [PCRP-ID1-2F11] followed by Goat Anti-Mouse IgG (CF® 488) (Green). CF® 640A Phalloidin (Red).

Cliquez pour ouvrir le point de vue élargi

Figure 3: SDS-PAGE - Anti-ID1 Antibody [PCRP-ID1-2F11] - BSA and Azide free (A278240)

SDS-PAGE analysis of Anti-ID1 Antibody [PCRP-ID1-2F11] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.

Cliquez pour ouvrir le point de vue élargi

Figure 4: Anti-ID1 Antibody [PCRP-ID1-2F11] - BSA and Azide free (A278240)

Analysis of protein array containing more than 19,000 full-length human proteins using Anti-ID1 Antibody [PCRP-ID1-2F11]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt^TM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt^TM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.