Anti-ICAM3 Anticorps [101-1D2] (A248909)

Anti-ICAM3 Antibody [101-1D2]

Mouse monoclonal [101-1D2] antibody to ICAM3.

This antibody recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (WS: IV V). Its epitope localizes in the D2 extracellular domain and is resistant to neuraminidase and proteases. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signalling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb inhibits primary mixed lymphocyte culture (MLC) but not secondary MLC, cytotoxicity or proliferation induced by mitogens. It blocks binding of NK1-L16 stimulated T cells to L cells expressing CD50. This MAb is excellent for staining of formalin/paraffin tissues.

Flow Cytometry, IF, IHC-P

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Human

Stimulated human leukocytes.

Mouse

Monoclonal

101-1D2

IgG2a

kappa

Protein A/G chromatography.

200 µg/ml

110-160 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-ICAM3 Antibody [101-1D2] - BSA and Azide free (A252089).

CD50, CDW 50, CDw50, ICAM 3, ICAM-3, ICAM-R, ICAM3_HUMAN, ICAMR, Intercellular adhesion molecule 3