Anti-Iba1 Anticorps (A83214)

Name: Anti-Iba1 Antibody
Brand: Antibodies.com
SKU: A83214
Price: 480.0 USD
Availability: InStock

Ce produit est identique à Abcam (ab48004). Découvrez plus pour moins cher.

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$480

Anticorps polyclonal de chèvre contre Iba1 pour ELISA, Flow Cytometry, IF et IHC.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 3-5 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Mots clés :

Microglia Marker

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Spécifications

Nom du produit

Anti-Iba1 Antibody

Description du produit

Goat polyclonal antibody to Iba1.

Applications

ELISA, Flow Cytometry, IF, IHC

Recommander des dilutions

ELISA: 1:8,000, IHC: 5-6µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivité

Human

Immunogène

Synthetic peptide corresponding to the internal region of human Iba1 (Isoform 3).

Séquence

C-NKQFLDDPKYSSDED

Hôte

Goat

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Forme du produit

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonymes

AIF 1, AIF-1, Aif1, AIF1 protein, AIF1_HUMAN, Allograft inflammatory factor 1, Allograft inflammatory factor 1 splice variant G, allograft inflammatory factor-1 splice variant Hara-1, balloon angioplasty responsive transcription, BART 1, G1, G1 putative splice variant of allograft inflamatory factor 1, IBA 1, interferon gamma responsive transcript, Interferon responsive transcript 1, interferon responsive transcript factor 1, Ionized calcium binding adapter molecule 1, ionized calcium-binding adapter molecule, Ionized calcium-binding adapter molecule 1, IRT 1, IRT1, Microglia response factor, MRF1, Protein g1

Contrôles isotype

Goat IgG (A121671)

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (8)

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Figure 1: Immunofluorescence - Anti-Iba1 Antibody (A83214)

Iba1 expression in U937 cells analyzed by immunofluorescence. Cells immobilized on Shifix™ coverslip and permeabilized with 0.15% Triton. Staining was performed with Anti-Iba1 Antibody (A83214) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and plasma membrane were stained and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 2: Immunofluorescence - Anti-Iba1 Antibody (A83214)

Iba1 expression in CaCo-2 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Iba1 Antibody (A83214) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and plasma membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Immunofluorescence - Anti-Iba1 Antibody (A83214)

Iba1 expression in U251 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Iba1 Antibody (A83214) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and plasma membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Flow Cytometry - Anti-Iba1 Antibody (A83214)

Iba1 expression in K562 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-Iba1 Antibody (A83214) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.