Human Vitamin D BP ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Vitamin D BP in the samples. A monoclonal antibody specific for human Vitamin D BP has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for Vitamin D BP, and Vitamin D BP present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Vitamin D BP bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Cell culture supernatant, serum and plasma.

Sensibilité

40.15 pg/ml

Gamme de detection

78.13 - 5000 pg/ml

Spécificité

This kit can assay both natural and recombinant human Vitamin D BP.

Reactivité

Human

Réactivité croisée

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at 2 - 8°C.

Synonymes

DBP, DBP/GC, GC, Gc globulin, Gc-globulin, GRD3, Group specific component, Group specific component vitamin D binding protein, Group-specific component, hDBP, VDB, VDBG, VDBP, Vitamin D binding alpha globulin, Vitamin D-binding protein, VTDB_HUMAN

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.