Human sTNF RII ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human sTNF RII in the samples. A monoclonal antibody specific for human sTNF RII has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for sTNF RII, and sTNF RII present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of sTNF RII bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Cell culture supernatant, serum and plasma.

Sensibilité

0.08 pg/ml

Gamme de detection

7.81 - 500 pg/ml

Récupération

113% (Average Recovery from Serum Samples).

Spécificité

This kit can assay both natural and recombinant human sTNF RII.

Reactivité

Human

Réactivité croisée

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at 2 - 8°C.

Synonymes

CD120b, p75, p75 TNF receptor, p75TNFR, p80 TNF alpha receptor, p80 TNF-alpha receptor, Soluble TNFR1B variant 1, TBP-2, TBPII, TNF R II, TNF R2, TNF R75, TNF-R2, TNF-RII, TNFBR, TNFR-II, TNFR1B, TNFR2, TNFR80, TNFRII, Tnfrsf1b, TNR1B_HUMAN, Tumor necrosis factor beta receptor, Tumor necrosis factor binding protein 2, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor type II, Tumor necrosis factor-binding protein 2

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.