Human TIM-1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human TIM-1 in the samples. A monoclonal antibody specific for human TIM-1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for TIM-1, and TIM-1 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of TIM-1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Cell culture supernatant, serum and plasma.

Sensibilité

1.42 pg/ml

Gamme de detection

31.25 - 2000 pg/ml

Récupération

102% (Average Recovery from Serum Samples).

Spécificité

This kit can assay both natural and recombinant human TIM-1.

Reactivité

Human

Réactivité croisée

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at 2 - 8°C.

Synonymes

CD365, HAVCR, HAVCR 1, HAVcr-1, Havcr1, Hepatitis A virus cellular receptor 1, Kidney injury molecule 1, KIM 1, KIM-1, T cell immunoglobin domain and mucin domain protein 1, T cell immunoglobulin mucin family member 1, T cell immunoglobulin mucin receptor 1, T-cell immunoglobulin and mucin domain-containing protein 1, T-cell membrane protein 1, TIM, TIM 1, TIM1, TIMD 1, TIMD-1, TIMD1, TIMD1_HUMAN

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.