This TIE2 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-TIE2 Antibody. Standards or samples are then added to the microtire plate wells and TIE2, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of TIE2 present in the sample, Anti-TIE2 Antibody (Biotin) is added to each well to "sandwich" the TIE2 immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TIE2 will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.

Sensibilité

< 5 pg/ml

Gamme de detection

156-10,000 pg/ml

Reactivité

Human

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at 2-8°C for 4 months or at -20°C for 8 months.

Synonymes

Angiopoietin 1 receptor, Angiopoietin-1 receptor, CD202b, CD202b antigen, EC 2.7.10.1, Endothelial tyrosine kinase, Endothelium specific receptor tyrosine kinase 2, hTIE 2, hTIE2, Hyk, p140 TEK, soluble TIE2 variant 1, soluble TIE2 variant 2, Tek, tek tyrosine kinase, TEK tyrosine kinase endothelial, TEK tyrosine kinase, endothelial, TIE 2, TIE2_HUMAN, Tunica interna endothelial cell kinase, Tyrosine kinase with Ig and EGF homology domains 2, Tyrosine kinase with Ig and EGF homology domains-2, Tyrosine protein kinase receptor TEK, Tyrosine protein kinase receptor TIE 2, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE 2, Tyrosine-protein kinase receptor TIE-2, Venous malformations multiple cutaneous and mucosal, venous malformations, multiple cutaneous and mucosal, VMCM, VMCM 1, VMCM1

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.