This Survivin enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-Survivin Antibody. Standards or samples are then added to the microtire plate wells and Survivin, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of Survivin present in the sample, Anti-Survivin Antibody (Biotin) is added to each well to "sandwich" the Survivin immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Survivin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.

Sensibilité

< 2 pg/ml

Gamme de detection

62.5-4000 pg/ml

Reactivité

Human

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at 2-8°C for 4 months or at -20°C for 8 months.

Synonymes

API4, Apoptosis inhibitor 4, Apoptosis inhibitor survivin, Apoptosis inhibitor4, Baculoviral IAP repeat containing 5, Baculoviral IAP repeat containing protein 5, Baculoviral IAP repeat-containing protein 5, BIRC 5, BIRC5, BIRC5_HUMAN, EPR 1, IAP4, Survivin variant 3 alpha, SVV, TIAP

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.