Human P-Selectin ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human sP-Selectin in the samples. A monoclonal antibody specific for human sP-Selectin has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for sP-Selectin, and sP-Selectin present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of sP-Selectin bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Cell culture supernatant, serum and plasma.

Sensibilité

1.11 pg/ml

Gamme de detection

78.13 - 5000 pg/ml

Récupération

96% (Average Recovery from Serum Samples).

Spécificité

This kit can assay both natural and recombinant human P-Selectin.

Reactivité

Human

Réactivité croisée

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at 2 - 8°C.

Synonymes

Antigen CD62, CD62, CD62 antigen like family member P, CD62 antigen-like family member P, CD62P, FLJ45155, GMP 140, GMP-140, GMP140, GMRP, Granule membrane protein 140, Granule membrane protein 140kDa, Granulocyte membrane protein, GRMP, LECAM 3, LECAM3, Leukocyte endothelial cell adhesion molecule 3 , Leukocyte-endothelial cell adhesion molecule 3, LYAM3_HUMAN, P selectin, PADGEM, Platelet activation dependent granule external membrane protein, Platelet activation dependent granule-external membrane protein, Platelet alpha granule membrane protein, PSEL, Selectin P, Selectin P (granule membrane protein 140kDa antigen CD62), Selectin P (granule membrane protein 140kDa, antigen CD62), SELP

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.