Human Osteoprotegerin ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human OPG in the samples. A monoclonal antibody specific for human OPG has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for OPG, and OPG present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of OPG bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Cell culture supernatant, serum and plasma.

Sensibilité

1.36 pg/ml

Gamme de detection

62.50 - 4000 pg/ml

Récupération

103% (Average Recovery from Serum Samples).

Spécificité

This kit can assay both natural and recombinant human OPG.

Reactivité

Human

Réactivité croisée

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at 2 - 8°C.

Synonymes

MGC29565 , MGC93568, OCIF , OPG, Osteoclastogenesis inhibitory factor, PDB5, TNF receptor superfamily member 11b, TNFRSF 11B, TNFRSF11B, TR 1, TR1, TR11B_HUMAN, Tumor necrosis factor receptor superfamily member 11B

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.