Humain NKB Kit Elisa (A2240)

$585
Frais de livraison
Date de livraison
Livraison sous 7-10 jours ouvrables.
Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com
Nom du produit
Human NKB ELISA Kit
Description du produit
Human NKB ELISA Kit is a competitive (quantitative) Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of human NKB in serum, plasma or other biological fluids.
Type de test
Competitive (quantitative)
Principe du test
Human NKB ELISA Kit employs the Competitive Enzyme Immunoassay (ELISA) technique for the quantitative measurement ofhumanNKB in serum, plasma or other biological fluids. A monoclonal antibody specific for NKB has been pre-coated onto a 96-well microtiter plate. During the incubation, NKB in the sample or standard competes with a fixed amount of Biotinylated-NKB. Following incubation, Biotinylated-NKB and free NKB in the sample or standard are washed from the plate. Avidin conjugated to Horseradish Peroxidase (Avidin-HRP) is then added to each well and incubated. TMB substrate solution is then added to each well, and after incubation, the enzyme-substrate reaction is terminated by the addition of sulfuric acid stop solution. The color change is measured spectrophotometrically at a wavelength of 450nm, and the concentration of NKB in the samples is then determined by comparing the O.D. 450nm of the samples to the standard curve. The O.D. 450nm is inversely proportional to the concentration of NKB in the sample.
Plate-forme
Pre-coated Microplate (12 x 8 well strips)
Méthode de détection
Colourmetric
Instrument
Colorimetric Microplate Reader
Type d'échantillon
Serum, plasma or other biological fluids.
Sensibilité
3.12 pg/ml
Gamme de detection
12.5-800 pg/ml
Durée du test
1 - 2.5 Hours
Reactivité
Human
Recovery
Sample Type n Range Average
Serum 5 80% - 102% 91%
EDTA Plasma 5 81% - 100% 90%
Heparin Plasma 5 80% - 89% 84%
Linearity
Sample Type 1:2 1:4 1:8 1:16
Serum (n=5) 87-91% 87-107% 74-101% 92-97%
EDTA Plasma (n=5) 90-105% 84-101% 90-101% 79-108%
Heparin Plasma (n=5) 84-95% 92-105% 82-105% 89-91%
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Notes générales
Information online is representative. Always refer to the Product Manual inside the kit.
Stockage
Store at +4°C. Do not use past expiration date!
Synonymes
Gamma tachykinin 3, Neurokinin B, Neurokinin B like protein, Neurokinin B protein, Neurokinin beta, Neurokinin-B, NeurokininB, Neuromedin K, Neuromedin-K, NeuromedinK, NKNB, Preprotachykinin B, PreprotachykininB, PRO 1155, PRO1155, TAC 3, TAC3, Tachykinin 3, Tachykinin3, TKNK_HUMAN, ZNEUROK 1, ZNEUROK1
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
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