Human MMP-8 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human MMP-8 in the samples. A monoclonal antibody specific for human MMP-8 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for MMP-8, and MMP-8 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of MMP-8 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Cell culture supernatant, serum and plasma.

Sensibilité

12.66 pg/ml

Gamme de detection

78.13 - 5000 pg/ml

Récupération

97% (Average Recovery from Serum Samples).

Spécificité

This kit can assay both natural and recombinant human MMP-8.

Reactivité

Human

Réactivité croisée

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at 2 - 8°C.

Synonymes

CLG 1, CLG1, Collagenase 1, Collagenase 1 neutrophil, HNC, Matrix metallopeptidase 8 (neutrophil collagenase), Matrix metalloprotease 8, Matrix metalloproteinase-8, MMP 8, MMP8, MMP8_HUMAN, Neutrophil collagenase, PMNL CL, PMNL collagenase, PMNL-CL, PMNLCL

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.