Human MMP-2 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human MMP-2 in the samples. A monoclonal antibody specific for human MMP-2 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for MMP-2, and MMP-2 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of MMP-2 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Cell culture supernatant, serum and plasma.

Sensibilité

69.14 pg/ml

Gamme de detection

1 - 64 ng/ml

Récupération

108% (Average Recovery from Serum Samples).

Spécificité

This kit can assay both natural and recombinant human MMP-2.

Reactivité

Human

Réactivité croisée

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at 2 - 8°C.

Synonymes

72 kDa gelatinase, 72 kDa type IV collagenase, 72kD type IV collagenase, CLG 4, CLG 4A , CLG4, CLG4A, Collagenase Type 4 alpha, Collagenase type IV A, EC 3.4.24.24, Gelatinase A, Gelatinase alpha , Gelatinase neutrophil , Matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase), Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase, Matrix metalloproteinase 2, Matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IV collagenase), Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase), Matrix metalloproteinase II, Matrix metalloproteinase-2, MMP 2, MMP II, MMP2, MMP2_HUMAN, MONA, Neutrophil gelatinase, PEX, TBE 1, TBE-1

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.