Human IL-9 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human IL-9 in the samples. A monoclonal antibody specific for human IL-9 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for IL-9, and IL-9 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-9 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Cell culture supernatant, serum and plasma.

Sensibilité

0.06 pg/ml

Gamme de detection

7.81 - 500 pg/ml

Récupération

104% (Average Recovery from Serum Samples).

Spécificité

This kit can assay both natural and recombinant human IL-9.

Reactivité

Human

Réactivité croisée

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at 2 - 8°C.

Synonymes

Cytokine P40, Homolog of mouse T cell and mast cell growth factor 40 , HP40, IL 9, IL9, IL9_HUMAN, Interleukin 9 , Interleukin-9, Mast cell growth factor , MCGF, Megakaryoblast growth factor, P40, p40 cytokine , p40 T cell and mast cell growth factor, T cell growth factor 3, T cell growth factor p40 , T-cell growth factor P40, TCGF 3

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.