Human IGF-1R ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human IGF-1R in the samples. A monoclonal antibody specific for human IGF-1R has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for IGF-1R, and IGF-1R present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IGF-1R bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Cell culture supernatant, serum and plasma.

Sensibilité

13.27 pg/ml

Gamme de detection

218.75 - 14000 pg/ml

Récupération

91% (Average Recovery from Serum Samples).

Spécificité

This kit can assay both natural and recombinant human IGF-1R.

Reactivité

Human

Réactivité croisée

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at 2 - 8°C.

Synonymes

CD221, CD221 antigen, IGF 1 receptor, IGF 1R, IGF I receptor, IGF-I receptor, IGF1 Receptor, IGF1R_HUMAN, IGFIR, IGFIRC, IGFR, Insulin like growth factor 1 receptor, Insulin like growth factor 1 receptor precursor, Insulin-like growth factor 1 receptor beta chain, Insulin-like growth factor I receptor, JTK13, MGC142170, MGC142172, MGC18216, Soluble IGF1R variant 1, Soluble IGF1R variant 2

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.