Sandwich ELISA using Anti-Human IgD Antibody [RM123] as the capture antibody (100ng/well), and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
ELISA of human immunoglobulins shows Anti-Human IgD Antibody [RM123] reacts to IgD from human plasma and IgD? from human myeloma. There is no cross reactivity with human IgG, IgM, IgA, or IgE. The plate was coated with 50ng/well of different immunoglobulins. 200ng/ml, 50ng/ml, or 10ng/ml of Anti-Human IgD Antibody [RM123] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
A titer ELISA using Anti-Human IgD Antibody [RM123]. The plate was coated with different amounts of human IgD (from plasma). A serial dilution of Anti-Human IgD Antibody [RM123] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Produits alternatifs à Anti-Humain IgD Anticorps [RM123] (A121347)