Human HLA G ELISA Kit is a indirect Enzyme-Linked Immunosorbent Assay designed for the in vitro quantitative determination of human HLA G in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Type de test

Indirect (quantitative)

Principe du test

Human HLA G ELISA Kit (A326887) employs the Indirect ELISA technique for the quantitative measurement of human HLA G in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.. The 96-well microtiter plate has been pre-coated with HLA G antigen. The samples and standards are added to the wells and incubated. After washing with wash buffer, Biotinylated detection antibody is added and incubated. After washing with wash buffer, HRP-Streptavidin is then added and incubated. Following another wash step, TMB substrate is added to visualize the HRP enzymatic reaction. TMB is catalysed by HRP to produce a blue-coloured product that changes to yellow after the addition of the acidic stop solution. The density of yellow is proportional to the amount of HLA G sample captured on the plate. The concentration of HLA G can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Plate-forme

Pre-coated Microplate (12 x 8 well strips)

Méthode de détection

Colorimetric

Instrument

Colorimetric Microplate Reader

Type d'échantillon

Serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Sensibilité

1.875 ng/ml

Gamme de detection

3.125-200 ng/ml

Durée du test

4 hours

Reactivité

Human

Recovery

Sample Type	n	Range	Average
Serum	10	88% - 104%	93%
EDTA Plasma	10	89% - 103%	97%
Heparin Plasma	10	92% - 99%	95%

Linearity

Sample Type	n	1:2	1:4	1:8
Serum	10	89-105%	92-100%	85-100%
EDTA Plasma	10	87-98%	86-101%	87-98%
Heparin Plasma	10	85-101%	86-100%	86-98%

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Notes générales

Information online is representative. Always refer to the Product Manual inside the kit.

Stockage

Store at +4°C. Do not use past expiration date!

Synonymes

B2 microglobulin, DADB-15K14.8, HLA 6.0, HLA class I histocompatibility antigen alpha chain G, HLA class I histocompatibility antigen, alpha chain G, HLA class I molecule, HLA G antigen, HLA G histocompatibility antigen class I G, HLA G3, HLA-G, HLA-G histocompatibility antigen, class I, HLA60, HLAG, HLAG_HUMAN, Major histocompatibility complex class I G, MHC class I antigen, MHC class I antigen G, MHC G, T-cell A locus, TCA

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.