Human HGF ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human HGF in the samples. A monoclonal antibody specific for human HGF has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for HGF, and HGF present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of HGF bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Cell culture supernatant, serum and plasma.

Sensibilité

1.99 pg/ml

Gamme de detection

31.25 - 2000 pg/ml

Récupération

95% (Average Recovery from Serum Samples).

Spécificité

This kit can assay both natural and recombinant human HGF.

Reactivité

Human

Réactivité croisée

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at 2 - 8°C.

Synonymes

DFNB39, F TCF, Fibroblast derived tumor cytotoxic factor, Hepatocyte growth factor, Hepatocyte growth factor (hepapoietin A; scatter factor), Hepatocyte growth factor beta chain, Hepatocyte growth factor precursor, Hepatopoietin A, Hepatopoietin-A, HGFB, HGF_HUMAN, HPTA, Lung fibroblast derived mitogen , OTTHUMP00000161349, OTTHUMP00000206710, OTTHUMP00000206711, OTTHUMP00000206712, OTTHUMP00000206713, OTTHUMP00000206730, Scatter factor, SF

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.