Human Growth Hormone ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Growth Hormone in the samples. A monoclonal antibody specific for human Growth Hormone has been immobilized onto microwells. Standard or samples are pipetted into the wells, and Growth Hormone present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for Growth Hormone is added to the wells. Following a wash to remove any unbound detect antibody, streptavidin-HRP is added. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Growth Hormone bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Cell culture supernatant, serum and plasma.
Sensibilité
0.62 pg/ml
Gamme de detection
62.50 - 4000 pg/ml
Récupération
105% (Average Recovery from Serum Samples).
Spécificité
This kit can assay both natural and recombinant human Growth Hormone.
Reactivité
Human
Réactivité croisée
This assay has shown no significant cross-reactivity.