Human Granzyme A ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Granzyme A in the samples. A monoclonal antibody specific for human Granzyme A has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for Granzyme A, and Granzyme A present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Granzyme A bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Cell culture supernatant, serum and plasma.

Sensibilité

0.31 pg/ml

Gamme de detection

15.63 - 1000 pg/ml

Récupération

112% (Average Recovery from Serum Samples).

Spécificité

This kit can assay both natural and recombinant human Granzyme A.

Reactivité

Human

Réactivité croisée

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at 2 - 8°C.

Synonymes

CTL tryptase, CTLA3, Cytolytic t cell and natural killer cell specific trypsin like serine protease, Cytotoxic T lymphocyte associated serine esterase 3, Cytotoxic T lymphocyte proteinase 1, Cytotoxic T-lymphocyte proteinase 1, Fragmentin 1, Fragmentin-1, GRAA_HUMAN, Granzyme 1 cytotoxic T lymphocyte associated serine esterase 3, Granzyme A (Cytotoxic T lymphocyte associated serine esterase 3; Hanukah factor serine protease), Granzyme A (granzyme 1, cytotoxic T lymphocyte associated serine esterase 3), Granzyme A precursor, Granzyme-1, GZMA, H factor, Hanukah factor serine protease, Hanukkah factor, HF, HFSP, TSP1

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.