Human Granulin ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human GRN in the samples. A monoclonal antibody specific for human GRN has been pre-coated onto a microplate. Standard, samples and HRP-linked detect antibody specific for GRN are pipetted into the wells and GRN present is bound by the immobilized antibody and detect antibody following incubation. After washing, substrate solution is added to the wells and color develops in proportion to the amount of GRN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Cell culture supernatant, serum and plasma.
Sensitivity
10.81 pg/ml
Product Range
125 - 8000 pg/ml
Specificity
This kit can assay both natural and recombinant human GRN.
Reactivity
Human
Cross Reactivity
This assay has shown no significant cross-reactivity.