Human Fas Ligand ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Fas Ligand in the samples. A monoclonal antibody specific for human Fas Ligand has been immobilized onto microwells. Standard or samples are pipetted into the wells, followd by the addition of biotin-linked monoclonal antibody specific for Fas Ligand, and Fas Ligand present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Fas Ligand bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Cell culture supernatant, serum and plasma.

Sensibilité

3.42 pg/ml

Gamme de detection

15.63 - 1000 pg/ml

Récupération

97% (Average Recovery from Serum Samples).

Spécificité

This kit can assay both natural and recombinant human Fas Ligand.

Reactivité

Human

Réactivité croisée

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at 2 - 8°C.

Synonymes

ALPS1B, Apoptosis (APO 1) antigen ligand 1, Apoptosis antigen ligand, Apoptosis antigen ligand 1, APT1LG1, APTL, CD178, CD178 antigen, CD95 L, CD95 ligand, CD95-L, CD95L, CD95L protein, Fas antigen ligand, Fas L, Fas ligand (TNF superfamily member 6), FASL, Fasl Fas ligand (TNF superfamily member 6), FASLG, Generalized lymphoproliferative disease, Gld, soluble form, TNF superfamily member 6, TNFL6_HUMAN, TNFSF6, Tumor necrosis factor (ligand) superfamily member 6, Tumor necrosis factor ligand superfamily member 6

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.