Human sE-Selectin ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human sE-Selectin in the samples. A monoclonal antibody specific for human sE-Selectin has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for sE-Selectin, and sE-Selectin present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of sE-Selectin bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Cell culture supernatant, serum and plasma.

Sensibilité

1.94 pg/ml

Gamme de detection

93.50 - 6000 pg/ml

Récupération

99% (Average Recovery from Serum Samples).

Spécificité

This kit can assay both natural and recombinant human E Selectin.

Reactivité

Human

Réactivité croisée

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at 2 - 8°C.

Synonymes

CD26E, CD62 antigen-like family member E, CD62E, CD62E antigen, E-selectin, ELAM , ELAM-1, ELAM1, Endothelial adhesion molecule 1, Endothelial leukocyte adhesion molecule 1, ESEL , LECAM2, Leukocyte endothelial cell adhesion molecule 2, Leukocyte-endothelial cell adhesion molecule 2, LYAM2_HUMAN, Sele, Selectin E

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.