Human CTGF ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human CTGF in the samples. A monoclonal antibody specific for human CTGF has been immobilized onto microwells. Standard or samples are pipetted into the wells, and CTGF present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked monoclonal antibody specific for CTGF is added to the wells. Following a wash to remove any unbound detect antibody, streptavidin-HRP is added. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of CTGF bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Cell culture supernatant, serum and plasma.

Sensibilité

2.31 pg/ml

Gamme de detection

62.50 - 4000 pg/ml

Récupération

103% (Average Recovery from Serum Samples).

Spécificité

This kit can assay both natural and recombinant human CTGF.

Reactivité

Human

Réactivité croisée

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at 2 - 8°C.

Synonymes

CCN 2, CCN family member 2, CCN2, Connective tissue growth factor, CTGF_HUMAN, Hcs 24, Hcs24, Hypertrophic chondrocyte specific protein 24, Hypertrophic chondrocyte-specific gene product 24, Hypertrophic chondrocyte-specific protein 24, IBP-8, IGF-binding protein 8, IGFBP-8, IGFBP8, Insulin like Growth Factor Binding Protein 8, Insulin-like growth factor-binding protein 8, MGC102839, NOV 2, NOV2

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.