Human sCD40L ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human sCD40L in the samples. A monoclonal antibody specific for sCD40L has been immobilized onto microwells. Standards or samples and HRP-linked monoclonal antibody specific for sCD40L are pipetted into the wells, and sCD40L present is bound by the immobilized antibody and detect antibody following incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of sCD40L bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Cell culture supernatant, serum and plasma.

Sensibilité

11.3 pg/ml

Gamme de detection

156.25 - 10000 pg/ml

Récupération

101% (Average Recovery from Serum Samples).

Spécificité

This kit can assay both natural and recombinant human sCD40L.

Reactivité

Human

Réactivité croisée

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at 2 - 8°C.

Synonymes

CD 40L, CD154, CD40 antigen ligand, CD40 ligand, CD40 ligand, soluble form, CD40-L, CD40LG, CD40L_HUMAN, gp39, hCD40L, HIGM1, IGM, IMD3, soluble form, T B cell activating molecule, T BAM, T cell antigen Gp39 , T-cell antigen Gp39, TNF related activation protein, TNF-related activation protein, TNFSF5, TrAP, Tumor necrosis factor (ligand) superfamily member 5, Tumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome), Tumor necrosis factor ligand superfamily member 5

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.