Humain CD18 Kit Elisa (A310316)

Frais de livraison
Date de livraison
Livraison sous 7-9 jours ouvrables.
Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com
Nom du produit
Human CD18 ELISA Kit
Description du produit
Human CD18 ELISA Kit is a 90 minute sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human CD18 in serum, plasma, and other biological fluids.
Type de test
Sandwich (quantitative)
Principe du test
Human CD18 ELISA Kit (A310316) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human CD18 in serum, plasma or other biological fluids. An antibody specific for CD18 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the CD18 present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-CD18 Antibody, which also binds the CD18 present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-CD18 Antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-CD18 Antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of CD18 captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of CD18 in the samples can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
Plate-forme
Pre-coated Microplate (12 x 8 well strips)
Méthode de détection
Colorimetric
Instrument
Colorimetric Microplate Reader
Type d'échantillon
Serum, plasma or other biological fluids.
Sensibilité
3.13 ng/L
Gamme de detection
50-800 ng/L
Durée du test
1h 30m
Reactivité
Human
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Notes générales
Information online is representative. Always refer to the Product Manual inside the kit.
Stockage
Store at +4°C. Do not use past expiration date!
Synonymes
95 subunit beta, Beta 2 (antigen CD18 (p95), CD 18, Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor), Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement component 3 receptor 3 and 4 subunit, Complement receptor C3 beta subunit, Complement receptor C3 subunit beta, Integrin beta 2, Integrin beta chain beta 2, Integrin beta-2, Integrin beta-2 (ITGB2), Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit), ITB2_HUMAN, ITGB2, LAD, LCAMB, Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C, Leukocyte cell adhesion molecule CD18, LFA 1, LFA1, Lymphocyte function associated antigen 1, Lymphocyte function associated antigen 1 (LFA1), MAC 1, MAC1, Macrophage antigen 1 (mac1) beta, MF17, MFI7, OTTHUMP00000115278, OTTHUMP00000115279, OTTHUMP00000115280, OTTHUMP00000115281, OTTHUMP00000115282
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Kit Components
Item Quantity Storage
Pre-Coated 96 Well Microplate 12 x 8 Well Strips +4°C
Standard Solution 500µl +4°C
Standard Diluent 3ml +4°C
Biotinylated Detection Antibody 1ml +4°C
Streptavidin-HRP 6ml +4°C
Wash Buffer (25X) 20ml +4°C
Substrate Solution A 6ml +4°C
Substrate Solution B 6ml +4°C
Stop Solution 6ml +4°C
Plate Sealers 5 Adhesive Strips -
Foil Pouch 1 Zip-Sealed Pouch -
Required Materials
The following materials are not included in the kit, but are required to run this assay:
  • Microplate reader capable of measuring absorbance at 450nm ± 10nm.
  • Squirt bottle, multichannel pipette reservoir, or automated microplate washer.
  • Graph paper or computer software capable of generating logarithmic functions.
  • Test tubes and Eppendorf tubes to prepare standards and samples.
  • Multi- and single-channel pipettes and sterile pipette tips.
  • Deionized or distilled water.
  • Absorbent paper towels.
  • 37°C incubator.
Important Notes
  • Samples cannot be diluted for use with this kit. Owing to the material used to prepare the kit, sample matrix interference may falsely depress the specificity and accuracy of the assay.
  • Sample concentrations should be estimated before being used in the assay. If the sample concentration is not within the range of the standard curve, users must contact us to determine the optimal sample for their experiments.
  • Samples containing Sodium Azide (NaN3) cannot be used in this assay. NaN3 inhibits the activity of Horseradish Peroxidase (HRP) which is used in the detection step.
Bring all reagents and samples to room temperature before use.
We recommend that all standards, controls, and samples be assayed in duplicate.
Step 1
Prepare all reagents, samples, and working standards as directed in the Product Manual.
Step 2
Determine the number of microplate strips required for the assay and insert them into the plate frame. Unused strips should be stored in the foil pouch at +4°C.
Step 3
Add 50μl of standard to the standard wells.
Step 4
Add 40μl of samples to the sample wells.
Step 5
Add 10μl of Biotinylated Detection Antibody to sample wells (but not to the standard wells).
Step 6
Add 50μl of Streptavidin-HRP to sample wells and standard wells (but not to the blank control well). Mix well. Cover with the adhesive plate sealer provided. Incubate for 60 minutes at 37°C.
Step 7
Remove the plate sealer and wash the plate 5 times with Wash Buffer. Soak wells with 300μl Wash Buffer for 30 seconds to 1 minute for each wash. For automated washing, aspirate or decant each well and wash 5 times with Wash Buffer. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Blot the plate onto paper towels or other absorbent material.
Step 8
Add 50μl of Substrate Solution A to each well and then add 50μl of Substrate Solution B to each well. Cover with a new adhesive plate sealer and incubate for 10 minutes at 37°C in the dark.
Step 9
Add 50μl of Stop Solution to each well. The color in the wells should change from blue to yellow immediately. Note: If the color in the wells is green or the color change does not appear uniform, gently tap the plate to ensure thorough mixing.
Step 10
Determine the optical density (O.D. value) of each well within 10 minutes, using a microplate reader set to 450 nm.

Produits alternatifs à Humain CD18 Kit Elisa (A310316)

Haut