Human CCL26 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Eotaxin-3 in the samples. A monoclonal antibody specific for human Eotaxin-3 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for Eotaxin-3, and Eotaxin-3 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Eotaxin-3 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Cell culture supernatant, serum and plasma.

Sensibilité

1.44 pg/ml

Gamme de detection

15.63 - 1000 pg/ml

Récupération

94% (Average Recovery from Serum Samples).

Spécificité

This kit can assay both natural and recombinant human Eotaxin-3.

Reactivité

Human

Réactivité croisée

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at 2 - 8°C.

Synonymes

C-C motif chemokine 26, CC chemokine IMAC, CCL 26, CCL26_HUMAN, Chemokine (C C motif) ligand 26, chemokine N1, Eotaxin 3, Eotaxin-3, IMAC, Macrophage inflammatory protein 4-alpha, MIP 4a, MIP 4alpha, MIP-4-alpha, MIP4a, MIP4alpha, SCYA 26, SCYA26, Small inducible cytokine A26, Small inducible cytokine subfamily A (Cys Cys) member 26, Small inducible cytokine subfamily A member 26, Small-inducible cytokine A26, Thymic stroma chemokine 1, Thymic stroma chemokine-1, TSC 1, TSC-1, TSC1

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.