Human CCL11 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Eotaxin in the samples. A monoclonal antibody specific for human Eotaxin has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for Eotaxin, and Eotaxin present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Eotaxin bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Cell culture supernatant, serum and plasma.

Sensibilité

3.14 pg/ml

Gamme de detection

15.63 - 1000 pg/ml

Récupération

90% (Average Recovery from Serum Samples).

Spécificité

This kit can assay both natural and recombinant human Eotaxin.

Reactivité

Human

Réactivité croisée

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at 2 - 8°C.

Synonymes

C-C motif chemokine 11, CCL 11 , CCL11_HUMAN, Chemokine (C-C motif) ligand 11, Chemokine C C motif ligand 11, Chemokine CC Motif Ligand 11, Chemokine ligand 11, Eosinophil Chemotactic P, Eosinophil chemotactic protein, Eotaxin, Eotaxin 1, eotaxin-1, MGC22554, SCYA 11 , SCYA11 , Small inducible cytokine A11 , Small inducible cytokine subfamily A (Cys Cys) member 11 , small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin), Small inducible cytokine subfamily A member 11 , Small-inducible cytokine A11

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.