Human Butyrylcholinesterase ELISA Kit is a sandwich (quantitative) Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Butyrylcholinesterase in serum, plasma or other biological fluids.

Type de test

Sandwich (quantitative)

Principe du test

Human Butyrylcholinesterase ELISA Kit employs the Sandwich Enzyme Immunoassay (ELISA) technique for the quantitative measurement of human Butyrylcholinesterase in human. An antibody specific for Butyrylcholinesterase has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Butyrylcholinesterase present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Butyrylcholinesterase Antibody, which binds the captured Butyrylcholinesterase present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and Avidin conjugated to Horseradish Peroxidase (Avidin-HRP) is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Butyrylcholinesterase captured in each well. The concentration of Butyrylcholinesterase can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Plate-forme

Pre-coated Microplate (12 x 8 well strips)

Méthode de détection

Colourmetric

Instrument

Colorimetric Microplate Reader

Type d'échantillon

Serum, plasma or other biological fluids.

Sensibilité

0.136 ng/ml

Gamme de detection

0.312-20 ng/ml

Durée du test

1 - 4.5 Hours

Reactivité

Human

Recovery

Sample Type	n	Range	Average
Serum	5	80% - 102%	91%
EDTA Plasma	5	81% - 100%	90%
Heparin Plasma	5	80% - 89%	84%

Linearity

Sample Type	1:2	1:4	1:8	1:16
Serum (n=5)	87-91%	87-107%	74-101%	92-97%
EDTA Plasma (n=5)	90-105%	84-101%	90-101%	79-108%
Heparin Plasma (n=5)	84-95%	92-105%	82-105%	89-91%

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Notes générales

Information online is representative. Always refer to the Product Manual inside the kit.

Stockage

Store at +4°C. Do not use past expiration date!

Synonymes

Acylcholine acylhydrolase, BCHE, Butyrylcholine esterase, CHE1, CHE2, CHLE_HUMAN, Choline esterase II, Cholinesterase, Cholinesterase (serum) 2, Cholinesterase 1, E1, Pseudocholinesterase, Pseudocholinesterase E1

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.