Human BDNF ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human BDNF in the samples. A monoclonal antibody specific for human BDNF has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for BDNF, and BDNF present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of BDNF bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Cell culture supernatant, serum and plasma.
Sensibilité
1.41 pg/ml
Gamme de detection
7.81 - 500 pg/ml
Récupération
101% (Average Recovery from Serum Samples).
Spécificité
This kit can assay both natural and recombinant human BDNF.
Reactivité
Human
Réactivité croisée
This assay has shown no significant cross-reactivity.