Human BAFF ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human BAFF in the samples. A monoclonal antibody specific for human BAFF has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for BAFF, and BAFF present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of BAFF bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Cell culture supernatant, serum and plasma.

Sensibilité

0.23 pg/ml

Gamme de detection

31.25 - 2000 pg/ml

Récupération

102% (Average Recovery from Serum Samples).

Spécificité

This kit can assay both natural and recombinant human BAFF.

Reactivité

Human

Réactivité croisée

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at 2 - 8°C.

Synonymes

ApoL related ligand TALL 1, B cell Activating Factor, B lymphocyte stimulator, B-cell-activating factor, BLyS, CD 257, CD257, CD257 antigen, Delta BAFF, Dendritic cell derived TNF like molecule, Dendritic cell-derived TNF-like molecule, DTL, DTL precursor, PRO738, soluble form, TALL 1, TALL-1, TALL1, THANK, TN13B_HUMAN, TNF and APOL related leukocyte expressed ligand 1, TNF homolog that activates apoptosis, TNF homolog that activates apoptosis NKFB and JNK., TNF- and APOL-related leukocyte expressed ligand 1, TNFSF13B, TNFSF20, TNLG7A, Tumor necrosis factor (ligand) superfamily member 13b, Tumor necrosis factor ligand 7A, Tumor necrosis factor ligand superfamily member 13b, Tumor necrosis factor ligand superfamily member 20, Tumor necrosis factor like protein ZTNF4, Tumor necrosis factor superfamily member 13B, UNQ401, ZTNF4

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.