Human Apolipoprotein E ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Apo E in the samples. A monoclonal antibody specific for human Apo E has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for Apo E, and Apo E present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Apo E bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Cell culture supernatant, serum and plasma.
Sensibilité
1.71 pg/ml
Gamme de detection
78.13 - 5000 pg/ml
Spécificité
This kit can assay both natural and recombinant human Apo E.
Reactivité
Human
Réactivité croisée
This assay has shown no significant cross-reactivity.