Human Apolipoprotein B ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Apo B in the samples. A monoclonal antibody specific for human Apo B has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for Apo B, and Apo B present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Apo B bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Cell culture supernatant, serum and plasma.
Sensibilité
74. pg/ml
Gamme de detection
312.50 - 20000 pg/ml
Spécificité
This kit can assay both natural and recombinant human Apo B.
Reactivité
Human
Réactivité croisée
This assay has shown no significant cross-reactivity.
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at 2 - 8°C.
Synonymes
Apo B-100, Apo B-48, APOB, ApoB 100 , ApoB 48, APOB_HUMAN, Apolipoprotein B (including Ag(x) antigen), Apolipoprotein B 100, Apolipoprotein B-48, Apolipoprotein B48, FLDB, LDLCQ4
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.