Humain Angiogenin Kit Elisa (A514)

$655
Frais de livraison
Date de livraison
Livraison sous 7-10 jours ouvrables.
Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com
Nom du produit
Human Angiogenin ELISA Kit
Type de test
Sandwich (quantitative)
Principe du test
This Angiogenin enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-Angiogenin Antibody. Standards or samples are then added to the microtire plate wells and Angiogenin, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of Angiogenin present in the sample, Anti-Angiogenin Antibody (Biotin) is added to each well to "sandwich" the Angiogenin immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Angiogenin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.
Sensibilité
< 12 pg/ml
Gamme de detection
78-5000 pg/ml
Reactivité
Human
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at 2-8°C for 4 months or at -20°C for 8 months.
Synonymes
ALS9, AMYOTROPHIC LATERAL SCLEROSIS, ANG, ANG 1, ANG I, ANG1, ANGI, Angiogenin ribonuclease RNase A family 5, Angiogenin ribonuclease RNase A family, 5, ANGI_HUMAN, Epididymis luminal protein 168, HEL168, MGC22466, MGC71966, Ribonuclease 5, Ribonuclease RNase A Family 5, Ribonuclease5, RNase 5, RNASE4, RNASE5
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

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