Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
Cardiovascular heat shock protein, cvHSP, DKFZp779D0968, FLJ32733, Heat shock 27kda protein family member 7, Heat shock protein beta 7, Heat shock protein beta-7, Hsp25 2, HSPB7_HUMAN, Hypothetical protein flj34956, RP11-5P18.6
Figure 1: Western Blot - Anti-HSPB7 Antibody (A307784)
Western blot analysis of extracts of various cell lines, using Anti-HSPB7 Antibody (A307784) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-HSPB7 Antibody (A307784) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-HSPB7 Antibody (A307784) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-HSPB7 Antibody (A307784) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
