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Anti-HSP27 (phospho Ser15) Antibody (A95124)

Western Blot - Anti-HSP27 (phospho Ser15) Antibody (A7110) - Antibodies.com Immunohistochemistry - Anti-HSP27 (phospho Ser15) Antibody (A7110) - Antibodies.com Western Blot - Anti-HSP27 (phospho Ser15) Antibody (A7110) - Antibodies.com
$190
Product Datasheet

Rabbit polyclonal antibody to HSP27 (phospho Ser15) for WB, IHC and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 6-8 business days.

  • Specifications

Name
Anti-HSP27 (phospho Ser15) Antibody
Description
Rabbit polyclonal antibody to HSP27 (phospho Ser15).
Specificity
This antibody detects endogenous levels of HSP27 only when phosphorylated at Ser15.
Applications
WB, IHC, ELISA
Dilutions
WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:20000
Reactivity
Human
Immunogen
Synthetic peptide derived from human HSP27 around the phosphorylation site of Ser15 (amino acids 5-54).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
22kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein beta-1, Heat shock protein family B member 1, HSP 27, HSP28, HspB1, SRP27, Stress-responsive protein 27
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-HSP27 (phospho Ser15) Antibody (A7110) - Antibodies.comEnlarge Image

Western Blot - Anti-HSP27 (phospho Ser15) Antibody (A95124)

Western blot analysis of lysates from HeLa cells treated with UV using Anti-HSP27 (phospho Ser15) Antibody. The left hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemistry - Anti-HSP27 (phospho Ser15) Antibody (A7110) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-HSP27 (phospho Ser15) Antibody (A95124)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-HSP27 (phospho Ser15) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Western Blot - Anti-HSP27 (phospho Ser15) Antibody (A7110) - Antibodies.comEnlarge Image

Western Blot - Anti-HSP27 (phospho Ser15) Antibody (A95124)

Western blot analysis of various cells using Anti-HSP27 (phospho Ser15) Antibody.
Publishing research using Anti-HSP27 (phospho Ser15) Antibody (A95124)? Please let us know so that we can list the citation on this page.

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