Rabbit polyclonal antibody to HSF1 (Phospho-Thr142)
Spécificité
The antibody detects endogenous levels of HSF1 only when phosphorylated at threonine 142.
Applications
IHC
Recommander des dilutions
Immunohistochemistry: 1:50~1:100
Reactivité
Human, Mouse
Immunogène
Peptide sequence around phosphorylation site of threonine 142(L-L-T(p)-D-V) derived from Human HSF1 .
Hôte
Rabbit
Clonalité
Polyclonal
Conjuguer
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0mg / ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunohistochemical analysis of paraffin-embedded human brain tissue using HSF1 (Phospho-Thr142) antibody #11715 (left)or the same antibody preincubated with blocking peptide (right).
Produits alternatifs à Anti-HSF1 (Phospho-Thr142) Anticorps (A51448)