Anti-HSF1 Anticorps [10H8] (A305101)

Name: Anti-HSF1 Antibody [10H8]
Brand: Antibodies.com
SKU: A305101
Price: 390.0 USD
Availability: InStock

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$390

Anticorps monoclonal [10H8] de rat contre HSF1 pour WB, IHC, ICC/IF, IP, ELISA et Gel Shift.

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Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

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Spécifications

Nom du produit

Anti-HSF1 Antibody [10H8]

Description du produit

Rat monoclonal [10H8] antibody to HSF1.

Applications

WB, IHC, ICC/IF, IP, ELISA, Gel Shift

Recommander des dilutions

WB: 1:1,000, IHC: 1:1,000, ICC/IF: 1:200

Reactivité

Human, Mouse, Rat, Bovine, Guinea Pig, Hamster, Monkey, Rabbit

Immunogène

Purified recombinant mouse HSF1 protein, with epitope mapping to amino acids 378-395.

Hôte

Rat

Clonalité

Monoclonal

Clone

10H8

Isotype

IgG1

Conjuguer

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Masse moléculaire

~85 kDa (unstressed cell lysates) / ~95 kDa (heat shocked cell lysates)

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

Heat shock factor 1, Heat shock factor protein 1, Heat shock transcription factor 1, HSF 1, HSF1_HUMAN, HSTF 1, HSTF1

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (5)

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Figure 1: Western Blot - Anti-HSF1 Antibody [10H8] (A305101)

Western blot analysis of human Breast adenocarcinoma cell line (MCF7) showing detection of ~65 kDa HSF1 protein using Anti-HSF1 Antibody [10H8] (A305101) at 1:1,000 for 2 hours at room temperature. Lane 1: MW ladder. Lane 2: HSF1 null lysate prepared from mouse embryonic fibroblasts. Lane 3: MCF7 lysate (5 µg). Lane 4: MCF7 lysate (10 µg). Lane 5: MCF7 lysate (20 µg). Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Goat Anti-Rat IgG: HRP for 1 hour at room temperature. Predicted/Observed Size: ~65 kDa.

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Figure 2: Immunohistochemistry - Anti-HSF1 Antibody [10H8] (A305101)

Immunohistochemistry analysis of human breast carcinoma, fixed in 10% formalin solution for 20 hours at room temperature. The Primary Antibody used was Anti-HSF1 Antibody [10H8] (A305101) at 1:1,000 for 40 min. The secondary antibody used was Dako labeled Polymer HRP Anti-rat IgG, DAB Chromogen (brown) (Dako Envision+ System) for 30 minutes at room temperature. Counterstain: Mayer's Hematoxylin (purple/blue) nuclear stain for 1 minute at room temperature. Localization: Nuclear. Magnification: 100X.

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Figure 3: Immunofluorescence - Anti-HSF1 Antibody [10H8] (A305101)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-HSF1 Antibody [10H8] (A305101), at 1:100 for 12 hours at 4°C. The secondary antibody used was APC Goat Anti-Rat (red) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-HSF1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

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Figure 4: Immunofluorescence - Anti-HSF1 Antibody [10H8] (A305101)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked mitotic HeLa cells, using Anti-HSF1 Antibody [10H8] (A305101), at 1:1,000. HSF1 stained green.