Anti-HP1 alpha Anticorps (A82447)

Name: Anti-HP1 alpha Antibody
Brand: Antibodies.com
SKU: A82447
Price: 480.0 USD
Availability: InStock

Ce produit est identique à Abcam (ab77256). Découvrez plus pour moins cher.

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$480

Anticorps polyclonal de chèvre contre HP1 alpha pour ELISA, WB, IF et Flow Cytometry.

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Garantie de Correspondance des Prix

Alternative Formats and ConjugatesAlternative Formats

Biotin

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 3-5 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Mots clés :

Heterochromatin Marker

Spécifications

Nom du produit

Anti-HP1 alpha Antibody

Description du produit

Goat polyclonal antibody to HP1 alpha.

Applications

ELISA, WB, IF, Flow Cytometry

Recommander des dilutions

ELISA: 1:128,000, WB: 0.03-0.1µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivité

Human

Immunogène

Synthetic peptide corresponding to the internal region of human HP1 alpha.

Séquence

C-NKRKSNFSNSADDIK

Hôte

Goat

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Masse moléculaire

24-25 kDa

MW prédit

22.2kDa

Forme du produit

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonymes

Antigen p25, CBX5, CBX5_HUMAN, CG8409, Chromobox 5, Chromobox homolog 5, Chromobox homolog 5 (HP1 alpha homolog, Drosophila), Chromobox protein homolog 5, Epididymis luminal protein 25, HEL25, Heterochromatin protein 1, Heterochromatin protein 1 alpha, Heterochromatin protein 1 homolog alpha, HP1, HP1 alpha homolog, HP1A, HP1Hs alpha, Su(var)205

Contrôles isotype

Goat IgG (A121671)

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

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Figure 1: Western Blot - Anti-HP1 alpha Antibody (A82447)

HP1 alpha expression in A43N (A) and K562 (B) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-HP1 alpha Antibody (A82447) at 0.03µg/ml and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-HP1 alpha Antibody (A82447)

HP1 alpha expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-HP1 alpha Antibody (A82447) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 4µg/ml. Nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 4µg/ml.

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Figure 3: Flow Cytometry - Anti-HP1 alpha Antibody (A82447)

HP1 alpha expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-HP1 alpha Antibody (A82447) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.