Horse POMC ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of horse POMC in serum, plasma, saliva or other biological fluids.

Type de test

Sandwich (quantitative)

Principe du test

Horse POMC ELISA Kit (A326186) employs the sandwich enzyme immunoassay technique for the quantitative measurement of horse POMC in serum, plasma, saliva or other biological fluids. An antibody specific for POMC has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the POMC present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-POMC Antibody, which binds the captured POMC present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of POMC captured in each well. The concentration of POMC can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Plate-forme

Pre-coated Microplate (12 x 8 well strips)

Méthode de détection

Colourmetric

Instrument

Colorimetric Microplate Reader

Type d'échantillon

Serum, plasma, saliva or other biological fluids.

Sensibilité

45 pg/ml

Gamme de detection

125-8000 pg/ml

Durée du test

4h 30m

Reactivité

Horse

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Notes générales

Information online is representative. Always refer to the Product Manual inside the kit.

Stockage

Store kit components at +4°C or -20°C as instructed. Do not use past expiration date!

Synonymes

ACTH, Adrenocorticotropic hormone, Adrenocorticotropin, alpha melanocyte stimulating hormone, Alpha MSH, Alpha-MSH, beta endorphin, Beta LPH, beta melanocyte stimulating hormone, Beta MSH, Beta-LPH, Beta-MSH, CLIP, COLI_HUMAN, Corticotropin like intermediary peptide, Corticotropin lipotropin, Corticotropin-lipotropin, Gamma MSH, Gamma-LPH, Gamma-MSH, Lipotropin beta, Lipotropin gamma, LPH, Melanotropin, Melanotropin alpha, Melanotropin beta, Melanotropin gamma, met enkephalin, Met-enkephalin, MSH, NPP, POC, pro ACTH endorphin, Proopiomelanocortin, Proopiomelanocortin preproprotein

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.