Anti-HMGB2 Anticorps [ARC1460] (A307358)

Name: Anti-HMGB2 Antibody [ARC1460]
Brand: Antibodies.com
SKU: A307358
Price: 470.0 USD
Availability: InStock

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$470

Anticorps monoclonal [ARC1460] de lapin contre HMGB2 pour WB, IHC, ICC/IF, IP et ChIP.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-HMGB2 Antibody [ARC1460]

Description du produit

Rabbit monoclonal [ARC1460] antibody to HMGB2.

Applications

WB, IHC, ICC/IF, IP, ChIP

Recommander des dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000, ChIP: 1:500-1:1,000, CUT&Tag: 1⁶ cells/1µg

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HMGB2 (P26583).

Séquence

MGKGDPNKPRGKMSSYAFFVQTCREEHKKKHPDSSVNFAEFSKKCSERWKTMSAKEKSKFEDMAKSDKARYDREMKNYVPPKGDKKGKKKDPNAPKRPPS

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC1460

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

24 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

C80539, High mobility group (nonhistone chromosomal) protein 2, High mobility group box 2, High mobility group protein 2, High mobility group protein B2, HMG 2, HMG B2, HMG-2, HMG2, HMGB2_HUMAN

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (9)

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Figure 1: Anti-HMGB2 Antibody [ARC1460] (A307358)

CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina from 10⁵ K562 cells with 1µg of Anti-HMGB2 Antibody [ARC1460] (A307358), and Goat Anti-Rabbit IgG H&L Antibody. The CUT&Tag results indicate the enrichment pattern of HMGB2 in representative gene loci (PTBP1), as shown in figure.

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Figure 2: Western Blot - Anti-HMGB2 Antibody [ARC1460] (A307358)

Western blot analysis of extracts of various cell lines, using Anti-HMGB2 Antibody [ARC1460] (A307358) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Immunohistochemistry - Anti-HMGB2 Antibody [ARC1460] (A307358)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-HMGB2 Antibody [ARC1460] (A307358) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-HMGB2 Antibody [ARC1460] (A307358)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-HMGB2 Antibody [ARC1460] (A307358) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.