Anti-HMGB1 Anticorps [ARC0001] (A308594)

Name: Anti-HMGB1 Antibody [ARC0001]
Brand: Antibodies.com
SKU: A308594
Price: 470.0 USD
Availability: InStock

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$470

Anticorps monoclonal [ARC0001] de lapin contre HMGB1 pour WB, IHC et ICC/IF.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Mots clés :

Anticorps Validé par Knockout

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Spécifications

Nom du produit

Anti-HMGB1 Antibody [ARC0001]

Description du produit

Rabbit monoclonal [ARC0001] antibody to HMGB1.

Applications

WB, IHC, ICC/IF

Recommander des dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB1 (P09429).

Séquence

SAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEKDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEEDEEDEEDEEEE

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC0001

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

25 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

Amphoterin, Chromosomal protein, nonhistone, HMG1, DKFZp686A04236, High mobility group 1, High mobility group box 1, High mobility group protein 1, High mobility group protein B1, high-mobility group (nonhistone chromosomal) protein 1, HMG-1, HMG1, HMG3, HMGB 1, HMGB1_HUMAN, NONHISTONE CHROMOSOMAL PROTEIN HMG1, SBP 1, Sulfoglucuronyl carbohydrate binding protein

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (8)

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Figure 1: Western Blot - Anti-HMGB1 Antibody [ARC0001] (A308594)

Western blot analysis of extracts of various cell lines, using Anti-HMGB1 Antibody [ARC0001] (A308594) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 2: Western Blot - Anti-HMGB1 Antibody [ARC0001] (A308594)

Western blot analysis of extracts from wild type (WT) and HMGB1 knockout (KO) 293T cells, using Anti-HMGB1 Antibody [ARC0001] (A308594) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 3: Western Blot - Anti-HMGB1 Antibody [ARC0001] (A308594)

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Figure 4: Immunohistochemistry - Anti-HMGB1 Antibody [ARC0001] (A308594)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-HMGB1 Antibody [ARC0001] (A308594) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.