Figure 1: Western Blot - Anti-Histone H2A.Z Antibody [ARC1048] (A306893)
Western blot analysis of extracts of various cell lines, using Anti-Histone H2A.Z Antibody [ARC1048] (A306893) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Figure 2: Western Blot - Anti-Histone H2A.Z Antibody [ARC1048] (A306893)
Western blot analysis of extracts from wild type(WT) and Histone H2A. Z knockout (KO) 293T(KO) cells, using Anti-Histone H2A.Z Antibody [ARC1048] (A306893) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Chromatin immunoprecipitation (ChIP) analysis of extracts of HeLa cells, using Anti-Histone H2A.Z Antibody [ARC1048] (A306893) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Produits alternatifs à Anti-Histone H2A.Z Anticorps [ARC1048] (A306893)