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Anti-His Tag Aptamer [6H7] (Biotin) (A320842)

1 Références
Secondary Structure - Anti-His Tag Aptamer [6H7] (Biotin) (A320842) - Antibodies.com
$730
Product Datasheet
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Frais de livraison
$40
Date de livraison
Livraison sous 30-45 jours ouvrables.

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Name
Anti-His Tag Aptamer [6H7] (Biotin)
Description
Synthetic DNA aptamer to His Tag [6H7] (Biotin).
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatmer Binding Conditions
Selection Buffer: pH 7.5, with 50mM Dipotassium Phosphate, 150mM Sodium Chloride, and 0.05% Tween 20.
Sequence
5'-dGpdCpdTpdApdTpdGpdGpdGpdTpdGpdGpdTpdCpdTpdGpdGpdTpdTpdGpdGpdGpdApdTpdTpdGpdGpdCpdCpdCpdCpdGpdGpdGpdApdGpdCpdTpdGpdGpdCp-3'
Aptamer Length
40 nt
Aptamer Molecular Weight (Da)
12,488.1 Da
Aptamer Affinity
Affinity (Kd) = 4.6 nM
Extinction Coefficient
370000 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-His Tag Aptamer [6H7] (Biotin) (A320842) is a replica of the aptamer described in PMID: 21538335.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 30-45 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Secondary Structure - Anti-His Tag Aptamer [6H7] (Biotin) (A320842) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Anti-His Tag Aptamer [6H7] (Biotin) (A320842)
Secondary structure of Anti-His Tag Aptamer [6H7] (Biotin) (A320842), with a dG of -2.075 kcal/mol.
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Aptamer-based downstream processing of his-tagged proteins utilizing magnetic beads
Références : Anti-His Tag Aptamer [6H7] (Biotin) (A320842)
Résumé :

Aptamers are synthetic nucleic acid-based high affinity ligands that are able to capture their corresponding target via molecular recognition. Here, aptamer-based affinity purification for His-tagged proteins was developed. Two different aptamers directed against the His-tag were immobilized on magnetic beads covalently. The resulting aptamer-modified magnetic beads were characterized and successfully applied for purification of different His-tagged proteins from complex E. coli cell lysates. Purification effects comparable to conventional immobilized metal affinity chromatography were achieved in one single purification step. Moreover, we have investigated the possibility to regenerate and reuse the aptamer-modified magnetic beads and have shown their long-term stability over a period of 6 months.

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