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Recombinant Anti-His Tag Anticorps [RMH01] (A121359)

ELISA - Anti-His Tag Antibody [RMH01] (A121359) - Antibodies.com ELISA - Anti-His Tag Antibody [RMH01] (A121343) - Antibodies.com Western Blot - Anti-His Tag Antibody [RMH01] (A121274) - Antibodies.com
Anticorps Recombinant
$510

Anticorps monoclonal [RMH01] de Human contre His Tag pour WB et ELISA.

Qualité 100 % Garantie
Garantie de Correspond­ance des Prix

Frais de livraison
$40
Date de livraison
Livraison sous 4-6 jours ouvrables.

Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com

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Anti-His Tag Anticorps [HIS.H8]
Anticorps monoclonal [HISH8] de souris contre HHHHHH tag pour Dot, ELISA, IP, IS et WB.
Nom du produit
Anti-His Tag Antibody [RMH01]
Description du produit
Recombinant human monoclonal [RMH01] antibody to His Tag.
Applications
WB, ELISA
Recommander des dilutions
ELISA: 0.1-2 µg/ml (Capture) and 0.01-0.5 µg/ml (Detection), WB: 0.1-1 µg/ml
Réactivité croisée
There is no cross reactivity with other endogenous protein.
Immunogène
Mixture containing peptide with His Tag (N terminal) and peptide with His Tag (C terminal).
Hôte
Human
Clonalité
Monoclonal
Clone
RMH01
Isotype
Chimeric
Conjuguer

Unconjugated

Purification
Protein A chromatography.
Concentration
1 mg/ml
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Notes générales
This antibody is a Human-Rabbit chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of Anti-His Tag Antibody [RM146], with the constant domain of Human IgG1. This antibody is recognized only by an Anti-Human IgG secondary antibody.
Synonymes
6 His epitope tag, 6X His tag, Hexa His tag, HHHHHH epitope tag, HHHHHH tag, Polyhistidine Tag
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
ELISA - Anti-His Tag Antibody [RMH01] (A121359) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Anti-His Tag Antibody [RMH01] (A121359)
Sandwich ELISA performed on different amounts of whole lysate of 293T cells transfected with a DNA construct encoding Histone H3 (K36M) protein (His tag), using 50µl/well of different concentrations of chimeric human Anti-His Tag Antibody [RMH01] as the capture antibody. Anti-H3K36M Antibody was used as the detection antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA - Anti-His Tag Antibody [RMH01] (A121343) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Anti-His Tag Antibody [RMH01] (A121359)
A titer ELISA performed on purified recombinant PCSK9 protein (His tag). A serial dilution of chimeric human Anti-His Tag Antibody [RMH01] was used as the primary antibody. An alkaline phosphatase conjugated anti-Human IgG as the secondary antibody.
Western Blot - Anti-His Tag Antibody [RMH01] (A121274) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Western Blot - Anti-His Tag Antibody [RMH01] (A121359)
Western blot of 293T cells transfected (+) or untransfected (-) with a DNA construct encoding Histone H3 (G34W) protein (His tag), using Anti-His Tag Antibody [RMH01] at 0.2µg/ml, followed by a HRP conjugated anti-human IgG secondary antibody.

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