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Recombinant Anti-His Tag Anticorps [RM146] (Biotin) (A121257)

ELISA - Anti-His Tag Antibody (Biotin) [RM146] (A121323) - Antibodies.com Western Blot - Anti-His Tag Antibody (Biotin) [RM146] (A121257) - Antibodies.com Immunohistochemistry - Anti-His Tag Antibody (Biotin) [RM146] (A121257) - Antibodies.com Immunohistochemistry - Anti-His Tag Antibody (Biotin) [RM146] (A121257) - Antibodies.com
Anticorps Recombinant
$510

Anticorps monoclonal [RM146] de lapin contre His Tag (Biotin) pour WB, IP, ICC, Flow Cytometry et IHC.

Qualité 100 % Garantie
Garantie de Correspond­ance des Prix

Frais de livraison
$40
Date de livraison
Livraison sous 4-6 jours ouvrables.

Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com

Nom du produit
Anti-His Tag Antibody [RM146] (Biotin)
Description du produit
Recombinant rabbit monoclonal [RM146] antibody to His Tag (Biotin).
Spécificité
This antibody recognizes recombinant proteins containing the 6 x His Tag or 10 x His Tag, fused to either the amino or carboxy terminus.
Applications
WB, IP, ICC, Flow Cytometry, IHC
Recommander des dilutions
WB: 0.1-0.5 µg/ml, ICC: 0.5-2 µg/ml, FC: 0.5-2 µg/ml, IHC: 0.1-1 µg/ml, ELISA: 0.01-0.5 µg/ml
Reactivité
All Species
Réactivité croisée
There is no cross reactivity with other endogenous protein in mammalian or bacteria cells.
Immunogène
Mixture containing peptide with His Tag (N terminal) and peptide with His Tag (C terminal).
Hôte
Rabbit
Clonalité
Monoclonal
Clone
RM146
Isotype
IgG
Conjuguer

Biotin

Purification
Protein A chromatography.
Concentration
1 mg/ml
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
6 His epitope tag, 6X His tag, Hexa His tag, HHHHHH epitope tag, HHHHHH tag, Polyhistidine Tag
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
ELISA - Anti-His Tag Antibody (Biotin) [RM146] (A121323) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Anti-His Tag Antibody [RM146] (Biotin) (A121257)
A titer ELISA performed on purified recombinat PCSK9 protein (His tag). A serial dilution of Anti-His Tag Antibody [RM146] (Biotin) was used as the primary antibody, followed by alkaline phosphatase conjugated Streptavidin.
Western Blot - Anti-His Tag Antibody (Biotin) [RM146] (A121257) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Western Blot - Anti-His Tag Antibody [RM146] (Biotin) (A121257)
Western blot of 293T cells transfected (+) or untransfected (-) with a DNA construct encoding Histone H3 (G34W) protein (His tag), using Anti-His Tag Antibody [RM146] (Biotin) at 0.2µg/ml, followed by a HRP conjugated Streptavidin.
Immunohistochemistry - Anti-His Tag Antibody (Biotin) [RM146] (A121257) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunohistochemistry - Anti-His Tag Antibody [RM146] (Biotin) (A121257)
Immunohistochemistry staining of naive HepG2 cells (Negative control) using Anti-His Tag Antibody [RM146] (Biotin).
Immunohistochemistry - Anti-His Tag Antibody (Biotin) [RM146] (A121257) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunohistochemistry - Anti-His Tag Antibody [RM146] (Biotin) (A121257)
Immunohistochemistry staining of 293T cells expressing His Tag nuclear protein X, using Anti-His Tag Antibody [RM146] (Biotin).

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