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Anti-Hemoglobin A1c Aptamer (Biotin) (A321036)

1 Références
Secondary Structure - Anti-Hemoglobin A1c Aptamer (Biotin) (A321036) - Antibodies.com
$730
Product Datasheet
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$40
Date de livraison
Livraison sous 30-45 jours ouvrables.

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Name
Anti-Hemoglobin A1c Aptamer (Biotin)
Description
Synthetic DNA aptamer to Hemoglobin A1c (Biotin).
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatamer Refolding Conditions
Heat to 85-90°C for 2 minutes then cool to room temperature prior to use.
Apatmer Binding Conditions
Optimal dilution of the aptamer to be determined through serial dilution experiments.
Sequence
5'-dTpdGpdGpdCpdApdGpdGpdApdApdGpdApdCpdApdApdApdCpdApdCpdApdTpdCpdGpdTpdCpdGpdCpdGpdGpdCpdCpdTpdTpdApdGpdGpdApdGpdGpdGpdGpdCpdGpdGpdApdCpdGpdGpdGpdGpdGpdGpdGpdGpdGpdCpdGpdTpdGpdGpdTpdCpdTpdGpdTpdGpdGpdTpdGpdCpdTpdGpdTp-3'
Aptamer Length
72 nt
Aptamer Molecular Weight (Da)
22,588.62 Da
Aptamer Affinity
Affinity (Kd) = 7.3 nM
Extinction Coefficient
694400 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-Hemoglobin A1c Aptamer (Biotin) (A321036) is a replica of the aptamer described in PMID: 26797251.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 30-45 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Secondary Structure - Anti-Hemoglobin A1c Aptamer (Biotin) (A321036) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Anti-Hemoglobin A1c Aptamer (Biotin) (A321036)
Secondary structure of Anti-Hemoglobin A1c Aptamer (Biotin) (A321036), with a dG of -3.301 kcal/mol.
PubMed LogoAfficher la publication
On-chip, aptamer-based sandwich assay for detection of glycated hemoglobins via magnetic beads
Références : Anti-Hemoglobin A1c Aptamer (Biotin) (A321036)
Résumé :

Diabetes can be diagnosed and monitored by measurement of the cutoff ratio between glycated hemoglobins (HbA1c) and total hemoglobin (Hb), which does not require a fasting blood sample and is less influenced by biological variations. In this study, we combined the advantages of the microfluidic system and the selected low-cost, stable and specific aptamers and developed an integrated, aptamer-based microfluidic system for automatic glycated hemoglobin measurements. The detection process of human whole blood can be totally automated in this integrated microfluidic system. According to the experimental results, when compared to conventional bench-top manual assays, reagent consumption was significantly reduced by 75%, and the analysis time was reduced from 3.5h to 30 min. Besides, the novelty in this research also lies in the simultaneously performed two parallel assays for detection of Hb and HbA1c in a single chip. Therefore, this sensitive and low-cost aptamer-based microfluidic system may become a promising tool for point-of -care diagnosis of diabetes.

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